Journal: iScience
Article Title: Butyrate ameliorates doxorubicin-induced heart failure by inhibiting cardiomyocyte ferroptosis through the gut-heart axis
doi: 10.1016/j.isci.2026.114754
Figure Lengend Snippet: NaB reduced bacterial translocation and LPS levels by enhancing the barrier function of the colonic and cardiac tissues (A) Histopathological damage of colonic tissue in the DOX+NaB, DOX +NaCl group by Hematoxylin and Eosin (H&E) staining (scale bars, 50 μm). (B and C) Representative images, quantitative analysis of Claudin-1 (B) and ZO-1 (C) of colonic tissue in the DOX+NaB, DOX+NaCl group by immunofluorescence (scale bars, 50 μm). (D) Representative images of goblet cell changes of colonic tissue in the DOX+NaB, DOX+NaCl group by AB-PAS staining (scale bars, 50 μm). (E) The mRNA expression levels of Muc2 and Tff3 in the colonic tissue via qRT-PCR assay in the DOX+NaB and DOX+NaCl groups. (F) Serum and cardiac tissue LPS concentrations in the DOX+NaB and DOX+NaCl groups via ELISA. (G and H) Representative images (G), quantitative analysis (H) of EUB338 bacterial probe count in cardiac tissue of the DOX+NaB and DOX+NaCl groups by FISH (scale bars, 20 μm). (I) The Pearson’s correlation analysis of EUB338 probe count and LVEF decrease rate in the DOX+NaB and DOX+NaCl groups. Statistical significance was determined using the simple linear regression of Correlation. (J) Representative images and quantitative analysis of connexin-43 and N-cadherin in cardiac tissue of the DOX+ NaB, DOX + NaCl group by immunofluorescence (scale bars, 50 μm). DOX+NaB and DOX+NaCl groups, n = 8 per group. Error bars represent the mean ± SEM. For normally distributed data with homogeneous variances, statistical analyses were performed using Student’s t tests for two groups and one-way ANOVA for multiple comparisons. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Article Snippet: Rabbit Claudin 1 Polyclonal antibody , proteintech , Cat# 28674-1-AP; RRID: AB_2881190.
Techniques: Translocation Assay, Staining, Immunofluorescence, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay
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